|
KMID : 0900220090160020121
|
|
대한비과학회지
2009 Volume.16 No. 2 p.121 ~ p.127
|
|
|
The Effect of Cigarette Smoking on the Expression of MMP-9, TIMP-1 and VEGF in Nasal Polyp Epithelial Cells and Fibroblasts
|
|
Ahn Yun-Suk
Kim Jun-Mo
Wang Jong-Hwan
Jang Yong-Ju
|
|
|
Abstract
|
|
|
Background and Objectives: It has been known that MMP-9, TIMP-1, and VEGF play an important role in the formation of nasal polyps (NPs). The aim of this study is to investigate the effect of smoking on the expression of MMP-9, TIMP-1 and VEGF in the epithelial cells and fibroblasts of NPs.
Materials and Methods:The epithelial cells and fibroblasts of NPs obtained from 10 patients who underwent endoscopic sinus surgery were cultured and used. The prepared CSE concentrations were 5%, 1%, 0.5% and 0.1%. In the control group, cells were cultured for 9 hours in the media containing 1 ml of AEGM and DMEM/F-12K. In the CSE group, cells were treated with 5%, 1%, 0.5% and 0.1% CSE for 9 hours. After washing with PBS, cells were cultured in medium for 24 hours. ELISA was performed to measure the secretion of MMP-9, TIMP-1 and VEGF proteins and real-time PCR was performed to assay their mRNAs.
Results: The production of MMP-9, TIMP-1 and VEGF proteins did not change significantly in comparison with the control group in epithelial cells and fibroblasts after exposure to CSE.
Conclusion: These results suggest that CSE does not appear to have an effect on the expression of MMP-9, TIMP-1 and VEGF for developing and growth of nasal polyposis.
|
|
|
KEYWORD
|
|
|
Nasal polyp·Smoking, CSE, Epithelial cell, Fibroblasts
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|